Research on the bioactivity of isoquercetin extracted from marestail on bladder cancer EJ cell and the mechanism of its occurrence

Figures & data

Table I. Main equipments and reagents.

Materials	Sources	Materials	Sources
Trihydroxymethyl aminomethanetrihydroxymethylamidogen methane	Huamei Biological Engineering Company	Automatic light microscope	German Zeiss
Trypsin, Isoquercetin	Sigma Corp.	Immunohistochemical kit	Beijing Sequoia Biological Technology Co Ltd
DMEM medium, Ham’s F-12 medium, fetal calf serum、DMSO	Gibco Corp.	FACS Vantage SETM flow cytometry	America BD Company
Disodium Ethylenediamine tetra acetic acid (EDTA), Boric acid	USB Corp.	High speed freezing centrifuge	American BECKMAN
CO2 incubator	America Forma	Automatic mixer	German EPPENDORF

Figure 1. HE dyeing of bladder cancer EJ cell.

Figure 2. EJ cell activity counted by trypan blue counting before and after isoquercetin treatment.

Table II. Cell activity after isoquercetin treatment at different time points (%).

	0 h	12 h	24 h	36 h	48 h
NC	98.81 ± 1.99	98.80 ± 2.07	97.36 ± 4.19	93.40 ± 4.50	93.39 ± 5.63
Que	98.89 ± 2.98	98.90 ± 3.16	97.39 ± 3.08	94.33 ± 6.60	94.50 ± 7.78

Figure 3. Distribution of bladder cancer EJ cell cycle analyzed by flow cytometry, before and after isoquercetin treatment.

Figure 4. IHC detection of STAT3 in human bladder cancer tissue.

Figure 5. IHC detection of the expression of STAT3 in human bladder cancer tissue.

Figure 6. STAT3 immunocytochemistry in human bladder cancer EJ cell.

Figure 7. PIAS3 immunocytochemistry in human bladder cancer EJ cell.