Clinical test on circulating tumor cells in peripheral blood of lung cancer patients, based on novel immunomagnetic beads

Figures & data

Figure 1. CTC enrichment and separation equipment. (A) The beaker containing 50mLPBS. (B) Peristaltic pump. (C) Horizontal table and self-made enrichment and separation container. (D) Vacuum pump. E: The beaker for receiving PBS pumped from D.

Figure 2. Recognition and indentation of CTCs. (A) and (B) original cell A549. (C) A549 that reacted with immunomagnetic antibodies. (D) cell A549 and residual white cell after immunomagnetic enrichment and separation.

Figure 3. Comparison of white cells on the slide with different parameters. (A) 20 rpm/min speed with 5° angle of incline. (B) 40 rpm/min speed with 5° angle of incline. (C) 20 rpm/min speed with 15° angle of incline. (D) 20 rpm/min speed with 15° angle of incline .

Table I. Number of CTCs under different parameters.

Angle of C	Rate of shaking of table (r/m)
	20	40	60	80
	Number of CTCs	Number of CTCs	Number of CTCs	Number of CTCs
5	86	82	77	63
15	85	80	70	54
20	78	71	59	40

Figure 4. Regression curve of A549.

Table II. Evaluation and test results of cell recoveries from samples of peripheral blood from healthy volunteers mixed with A549.

Number of cells mixed	Number of recovered cells			Recovery
	Mean value	SD value	95% CI	Mean value	95% CI	% CV
50	36	7	30–41	71	68–74	18
100	82	12	72–92	82	77–87	14
200	146	14	137–162	75	68–81	10
500	368	27	345–391	74	62–86	7
1000	684	44	645–722	68	49–88	6

Figure 5. CTCs captured in the peripheral blood of lung cancer patients through the novel immunomagnetic separation method, and white cells around the CTCs, in the slide observed under the 40 × light microscope.

Table III. Results of CTCs detected according to the clinical features of lung cancer patients.