Figures & data
Figure 1. Schematic diagram: Mechanisms involved in SeNPs synthesis from keratin molecules.
Figure 2. Scanning electron microscope (SEM) analysis of the SeNPs. (A) SEM image of the SeNPs produced by keratin (20,000 X magnification) (B) Particles size analysis at 30,000 X magnification (C) EDX analysis of ratio between thiol and SeNPs.
Figure 3. Viability of zebrafish embryos exposed to various concentrations of 100–200-nm SeNPs and sodium selenite after 96 hpf.
Figure 4. Zebrafish embryos exposed to different concentrations of 100–200-nm SeNPs. (A- control; B-5 μg/ml; C- 10 μg/ml; D-15 μg/ml; E- 20 μg/ml; F- 25 μg/ml).
Figure 5. Malformations (e.g., pericardial edema) induced by SeNPs (A) Control; (B) 5 μg/ml; (C) 10 μg/ml; (D) 15 μg/ml; (E) 20 μg/ml; and (F) 25 μg/ml.
Figure 6. Percentage of malformation (tail, pericardial edema) induced by different concentrations of SeNPs (100–200nm) and sodium selenite.
Figure 7. Zebrafish embryos exposed to different concentrations of sodium selenite. (A- control; B-5 μg/ml; C- 10 μg/ml; D-15 μg/ml; E- 20 μg/ml; F- 25 μg/ml).
Figure 8. Sodium-selenite-treated zebrafish embryo. (A) Control; (B) 5 μg/ml of sodium selenite; (C) 10 μg/ml of sodium selenite; (D) 15 μg/ml of sodium selenite; (E) 20 μg/ml of sodium selenite; (F) 25 μg/ml of sodium selenite.
Figure 9. Apoptosis assay of whole embryo: (A) Control; (B) 25 μg/ml of sodium selenite; (C) 25 μg/ml of SeNPs.
Figure 10. Effects of sodium selenite and SeNPs on heart rates of zebrafish embryos at 96 hpf. Differences were considered statistically significant with P value < 0.0001.
