The effect of cationic starch on hemoglobin, and the primary attempt to encapsulate hemoglobin

Figures & data

Figure 1. (a) The zeta potential of 0.25% cationic starch, and (b) the zeta potential of the mixture (0.25% cationic starch and 1 mg/mL hemoglobin).

Table I. The zeta potential and the size at main peak of the mixture (0.25% cationic starch and 1 mg/mL hemoglobin) at different pH values.

Initial pH	0.5 mol/L NaOH(μl)	Instant pH	Balanced pH	Zeta potential(mv)	Size at main peak(nm)
4.29	12	8.33	8.18	16.30 ± 1.27	186.2
4.28	10	8.34	7.92	20.70 ± 1.62	155.6
4.27	8	7.24	7.22	25.80 ± 2.02	138.7
4.30	7	6.68	6.82	16.60 ± 1.30	164.6
4.36	5	5.92	6.32	26.00 ± 2.04	201.1
4.35	0	4.35	4.35	34.20 ± 3.10	351.2

Figure 2. (a) The mixture (0.25% cationic starch and 1 mg/mL hemoglobin) at different pH value (8.18, 7.92, 7.22, 6.82 and 6.32 from left to right), (b) UV–Vis absorption spectrum of cationic starch and hemoglobin at pH 8.18, (c) UV–Vis absorption spectrum of cationic starch and hemoglobin at pH 7.92 and 7.22, (d) UV–Vis absorption spectrum of cationic starch and hemoglobin at pH 6.82, 6.32 and 4.35, (e) UV–Vis absorption spectrum of cationic starch and hemoglobin at above all pH value.

Figure 3. TEM pictures of cationic starch encapsulated hemoglobin without metal spraying under the multiple of 10 × 105. Cationic starch encapsulated bovine hemoglobin were dispersed in PBS solution and respectively dropped (a) immediately and (b) 1 h later on carbon support film.

Figure 4. Cationic starch encapsulated bovine hemoglobin were dispersed in a PBS solution. Its size was measured by non-invasive back scatter in Malvern Nano-zs 90 (a) immediately, (b) 1 h later and (c) each 10 min.