Cartilage-derived extracellular matrix extract promotes chondrocytic phenotype in three-dimensional tissue culture

Figures & data

Figure 1. Experimental design and composition of hydrogel constructs.

Figure 2. CDCs in monolayer (A) and hydrogel (B) culture, and quantification of MSC and CDC CFUs (C). Scale bars are equivalent to 200 μm (A) and 22 mm (B).

Table I. Custom qPCR primers were used to evaluate the efficacy of chondrogenesis.

Target	Forward	Reverse
β-ACTIN	AGTGTGACGTGGACATCCG	AATCTCATCTTGTTTTCTGCGC
SOX 9	CAACGCCGAGCTCAGCA	TCCACGAAGGGCCGC
AGC	TCGAGGACAGCGAGGCC	TCGAGGGTGTAGCGTGTAGAGA
ALK	GCAACCTGCAGTGTTGCATC	CGGATCTGCTCGTCCAGCAC
COL I	AGGGCTCCAACGAGATCGAGATCCG	TACAGGAAGCAGACAGGGCCAACG
COL II	GGCAATAGCAGGTTCACGTACA	CGATAACAGTCTTGCCCCACTT
COL X	CAAGGCACCATCTCCAGGAA	AAAGGGTATTTGTGGCAGCATATT

Figure 3. Proliferation of MSCs (A) and CDCs (B) over time.

Figure 4. Histological cross-sections of hydrogel constructs. Scale bars are equivalent to 200 μm.

Figure 5. Gene expression of MSCs following 19 days in hydrogel culture.

Figure 6. Gene expression of CDCs following 19 days in hydrogel culture.