Polymerized human placenta hemoglobin improves resuscitative efficacy of hydroxyethyl starch in a rat model of hemorrhagic shock

Figures & data

Table I. Properties of PolyPHb and HES solutions.

	PolyPHb	HES 130
Concentration (g/dl)	(Hb) 6	(HES) 6
Average molecular weight (kDa)	220 (64–600)	130 (110–150)
P50 (mmHg)	11	–
Oxygen-carrying capacity (ml/gHb)	1.19	–
COP (mmHg)	5.5	36
Suspending fluid	Normal saline	Normal saline

PolyPHb, Polymerized human placenta hemoglobin; HES, hydroxyethyl starch; COP, colloid osmotic pressure.

Figure 1. Volume-controlled hemorrhagic shock model in Sprague–Dawley rats. Hemorrhage (60% of BV) was completed by two steps: rapid hemorrhage (30% of BV) from 0 to 10 min and slow hemorrhage (30% of BV) from 10 to 50 min; resuscitation with PolyPHb/HES (in a 1:2 ratio) and HES 130 alone solutions from 60 to 75 min; and the observation until 72 h.

Figure 2. Hematocrit of rat arterial blood at baseline, during shock and after resuscitation. Data were presented as Mean ± SD (n = 7–8).

Figure 3. Survival rate of Sprague–Dawley rats after resuscitation from hemorrhagic shock with infusion of PolyPHb/HES (in a 1:2 ratio) and HES 130/0.4 alone.

Table II. Mean arterial pressure (MAP) and heart rate (HR).

				Time after resuscitation
Time points		Baseline	Shock	0 min	30 min	60 min
MAP (mmHg)	HES 130	125 ± 7	30 ± 5	93 ± 8	91 ± 6	90 ± 6*
	PolyPHb/HES	125 ± 5	29 ± 7	111 ± 6^#	106 ± 6	105 ± 8*^#
HR (beats/min)	HES 130	451 ± 17	372 ± 21	383 ± 21	412 ± 28	446 ± 22
	PolyPHb/HES	446 ± 19	370 ± 31	385 ± 16	407 ± 30	449 ± 21

Data were presented as Mean ± SD (n = 6–8). *p < 0.05 vs. baseline, ^#p < 0.05 vs. the HES 130 group at the same time point.

Table III. Arterial blood gas during the experiment.

	Group	Baseline	Shock	60 min after resuscitation
PaO2 (mmHg)	HES 130	77 ± 7	107 ± 10	106 ± 12*
	PolyPHb/HES	74 ± 4	104 ± 9	93 ± 9*^#
PaCO2 (mmHg)	HES 130	48 ± 6	34 ± 6	34 ± 4*
	PolyPHb/HES	50 ± 4	35 ± 7	38 ± 8*
BE (mmol/L)	HES 130	3.7 ± 1.6	− 12.6 ± 3.2	0.6 ± 2.2*
	PolyPHb/HES	3.6 ± 1.4	− 12.5 ± 2.6	0.4 ± 2.3*
pH	HES 130	7.38 ± 0.02	7.28 ± 0.06	7.38 ± 0.02
	PolyPHb/HES	7.38 ± 0.02	7.29 ± 0.04	7.40 ± 0.03

Data were presented as Mean ± SD (n = 7–8). *p < 0.05 vs. baseline, ^#p < 0.05 vs. the HES 130 group at the same time point.

Figure 4. Subcutaneous tissue oxygen pressure (PtO₂) during experiment. PtO₂ values (mmHg) were presented as Mean ± SD (n = 6). ^#P < 0.05 vs. the HES 130 group at the same time point.

Figure 5. Plasma EPO levels at different time points after resuscitation. EPO levels (mIU/ml) were presented as Mean ± SD (n = 4–6). *p < 0.05 vs. 0 h after resuscitation, ^#p < 0.05 vs. the PolyPHb/HES group at the same time point.