Extraction of superoxide dismutase, catalase, and carbonic anhydrase from stroma-free red blood cell hemolysate for the preparation of the nanobiotechnological complex of polyhemoglobin–superoxide dismutase–catalase–carbonic anhydrase

Figures & data

Table I. Recovery yield of apparent activities of CAT, SOD, and CA after extraction using different concentrations of phosphate ions (see Discussion, regarding the possible reasons for the higher recovery of CAT activity).

	4M PPB	3M PPB	2M PPB	1M PPB	0.1M PPB	0.05M PPB	Water	Nothing
Final PO4^2 −Concentration (mM)	507.8	382.8	257.8	132.8	20.3	14.05	7.8	9.375
CAT recovery yield (%)	194 ± 41	209 ± 65	204 ± 56	118 ± 23	6 ± 3	3 ± 2	0	11 ± 7
SOD recovery (%)	86 ± 7	88 ± 5	70 ± 10	70 ± 3	77 ± 9	80 ± 8	98 ± 9	80 ± 12
CA recovery (%)	80 ± 10	102 ± 5	74 ± 7	76 ± 5	84 ± 2	84 ± 7	95 ± 4	93 ± 10

Figure 1. (A) The molecular weight distribution of polySFHb–SOD–CAT–CA, or polySFHb (B) The molecular weight distribution of SFHb.

Figure 2. Comparison of CAT activities in different samples. Left to right: SFHb before and after crosslinking; SFHb + CAT+ SOD + CA 2x (SFHb plus 2x extracted SOD, CAT, and CA) before and after crosslinking; SFHb + CAT+ SOD + CA 4x (SFHb plus 4x extracted SOD, CAT, and CA) before and after crosslinking; SFHb + CAT+ SOD + CA 6x (SFHb plus 6x extracted SOD, CAT, and CA) before and after crosslinking.

Figure 3. Comparison of SOD activities in different samples. Left to right: SFHb before and after crosslinking; SFHb+ CAT+ SOD+ CA 2x (SFHb plus 2x extracted SOD, CAT, and CA) before and after crosslinking; SFHb+ CAT+ SOD+ CA 4x (SFHb plus 4x extracted SOD, CAT, and CA) before and after crosslinking; SFHb+ CAT+ SOD+ CA 6x (SFHb plus 6x extracted SOD, CAT, and CA) before and after crosslinking.

Figure 4. Comparison of CA activities in different samples. Left to right: SFHb before and after crosslinking; SFHb+ CAT+ SOD+ CA 2x (SFHb plus 2x extracted SOD, CAT, and CA) before and after crosslinking; SFHb+ CAT+ SOD+ CA 4x (SFHb plus 4x extracted SOD, CAT, and CA) before and after crosslinking; SFHb+ CAT+ SOD+ CA 6x (SFHb plus 6x extracted SOD, CAT, and CA) before and after crosslinking.