Down regulation of miR-18a, miR-21 and miR-221 genes in gastric cancer cell line by chrysin-loaded PLGA-PEG nanoparticles

Figures & data

Figure 1. (A) FTIR spectra of PLGA-PEG, (B) FTIR result shows the presence of chrysin in PLGA-PEG nanoparticle.

Figure 2. The¹H-NMR spectrum of PLGA-PEG.

Figure 3. (A) The micrographs of PLGA-PEG copolymers and (B) the micrographs of PLGA-PEG-loaded chrysin.

Table 1. IC50 value of pure and nano chrysin.

	Pure chrysin (μM)		Nano-chrysin (μM)
IC50	24 h	68.24	58.24
	48 h	56.16	44.21
	72 h	42.32	36.8

Figure 4. Real-time PCR results of miRNAs when treatment with pure chrysin. Real-time PCR results showed that relative expression of (A) miR-18a, (B) miR-21, and (C) miR-221 was decreased by pure chrysin.

Figure 5. Real-Time PCR results of miRNAs when treatment with nanochrysin. (A) Real-time PCR results showed that relative expression of (A) miR-18a, (B) miR-21, and (C) miR-221 was decreased by nanochrysin.