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Artificial Cells, Nanomedicine, and Biotechnology
An International Journal
Volume 45, 2017 - Issue 3
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Original Articles

Preparation and characterization of PLGA-β-CD polymeric nanoparticles containing methotrexate and evaluation of their effects on T47D cell line

Fatemeh GorjikhahDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; ;Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran; ;Department of Medical Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran;
,
Farid Azizi JalalianDepartment of Medical Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran;
,
Roya SalehiDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; ;Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran;
,
Yunes PanahiChemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;
,
Arash HasanzadehLaboratory of Biochemistry, Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran;
,
Effat AlizadehDepartment of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
,
Abolfazl AkbarzadehDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; ;Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran; Correspondence[email protected]
&
Soodabeh DavaranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; ;Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran; Correspondence[email protected]
 show all
Pages 432-440 | Received 15 Dec 2015, Accepted 27 Feb 2016, Published online: 22 Mar 2016

Figures & data

Figure 1. Chemical structure of PLGA-β-CD copolymer.

Figure 1. Chemical structure of PLGA-β-CD copolymer.

Figure 2. 1H-NMR spectrum of PLGA-β-CD copolymer.

Figure 2. 1H-NMR spectrum of PLGA-β-CD copolymer.

Figure 3. Fourier transform infrared spectroscopy (FT-IR) of pure methotrexate.

Figure 3. Fourier transform infrared spectroscopy (FT-IR) of pure methotrexate.

Figure 4. Fourier transform infrared spectroscopy (FT-IR) of poly (lactic-co-glycolic acid) (PLGA)-beta-cyclodextrin (βC) copolymer.

Figure 4. Fourier transform infrared spectroscopy (FT-IR) of poly (lactic-co-glycolic acid) (PLGA)-beta-cyclodextrin (βC) copolymer.

Figure 5. Fourier transform infrared spectroscopy (FT-IR) of methotrexate-poly (lactic-co-glycolic acid) (PLGA)-beta-cyclodextrin (βC) copolymer.

Figure 5. Fourier transform infrared spectroscopy (FT-IR) of methotrexate-poly (lactic-co-glycolic acid) (PLGA)-beta-cyclodextrin (βC) copolymer.

Figure 6. Scanning electron microscopy (SEM) of nanoparticles surface fabricated by PLGA-β-CD(I) with mtx.

Figure 6. Scanning electron microscopy (SEM) of nanoparticles surface fabricated by PLGA-β-CD(I) with mtx.

Figure 7. Cytotoxicity effect of PLGA-βC methotrexate complex and free methotrexate on T47D for 24 h. *: P values < 0.05 was considered statistically significant.

Figure 7. Cytotoxicity effect of PLGA-βC methotrexate complex and free methotrexate on T47D for 24 h. *: P values < 0.05 was considered statistically significant.

Figure 8. Cytotoxicity effect of PLGA-βC methotrexate complex and free methotrexate on T47D for 48 h. *: P values < 0.05 was considered statistically significant.

Figure 8. Cytotoxicity effect of PLGA-βC methotrexate complex and free methotrexate on T47D for 48 h. *: P values < 0.05 was considered statistically significant.

Figure 9. Cytotoxicity effect of PLGA-βC methotrexate complex and free methotrexate on T47D for 72 h. *: P values < 0.05 was considered statistically significant.

Figure 9. Cytotoxicity effect of PLGA-βC methotrexate complex and free methotrexate on T47D for 72 h. *: P values < 0.05 was considered statistically significant.

Figure 10. Cytotoxicity study of free drug and drug loaded nanoparticles on T47D cell line after, 24 h (a), 48 h (b), and 72 h (c) exposure.

Figure 10. Cytotoxicity study of free drug and drug loaded nanoparticles on T47D cell line after, 24 h (a), 48 h (b), and 72 h (c) exposure.

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