Farnesol altered morphogenesis and induced oxidative burst–related responses in Rhizoctonia solani AG1-IA

ABSTRACT

Farnesol induces morphological changes characteristic of apoptosis in filamentous fungi. Growth-inhibitory effect and induced features of apoptosis on Rhizoctonia solani AG1-IA were observed in our study by addition of exogenous farnesol to the culture. The obtained results implied that farnesol triggered apoptosis-like features, such as production of reactive oxygen species (ROS), in R. solani AG1-IA and that there was increased superoxide dismutase (SOD) activity in the presence of farnesol, as well as decreased fungal biomass. Light microscopic analysis showed that farnesol disrupted the cytoplasm and deformed the hyphae of R. solani AG1-IA. The diameter of the hyphal cross-section in the fungus treated with farnesol decreased compared with control. Transmission electron microscopy (TEM) showed marked alternations in the cell wall, cell membrane, parenthesome, septum, and septal pore of the fungal cells. The findings of this work suggest that farnesol is deleterious to R. solani and has potential for use as an antifungal compound against this destructive phytopathogenic fungus.

KEYWORDS:

• Cell death
• electron microscopy
• quorum sensing molecule
• reactive oxygen species
• superoxide dismutase
• Thanatephorus cucumeris

Additional information

Funding

We thank Ferdowsi University of Mashhad, Iran, for financial support of this research with project number 3/43459 approved on 3 April 2017.