Nano titanium exposure induces dose- and size-dependent cytotoxicity on human epithelial lung and colon cells

Abstract

The productions as well as use of Titanium dioxide nanoparticles (TNPs) were rapidly increasing in the present nano-world. The TNP becomes an inevitable part our daily life in the form of cosmeceutical, bio-medical, and nano-pharmaceutical applications. The TNPs are either inhaled or ingested into the human body through common routes of exposure like the lungs and the oral-gastrointestinal tract (GIT). Human lung and colon were exposed to test particles, TNP 18 nm (TNP 18), TNP 30 nm (TNP 30), and TNP 87 nm (TNP 87) with a dose range 0.1–100 µg/ml. The effect of exposure was determined using MTT, LDH, and DCFH-DA methods. The TNP 18, TNP 30, and TNP 87 significantly (p < 0.001) reduced cell viability in a dose- and a size-dependent manner in 60 and 100 µg/ml. The lowest IC₅₀ values 21.80 and 24.83 µg/ml were observed in A549 and Caco-2 for the smallest size, TNP 18. Further, for TNP 30, IC₅₀ values were 23.30 and 28.59 µg/ml compared to Nano QTZ 43.82 and 45.86 µg/ml. The EC₂₅ values of LDH leakage were 5.83 and 9.50 µg/ml for TNP 18 in lung and colon cells. Besides, ROS levels increased significantly at doses 60 (p < 0.01) and 100 (p < 0.001) µg/ml in two cells. The smaller size particle, TNP 18 has produced a significant (p < 0.05) toxic effect at the lowest dose i.e., 10 µg/ml. Therefore, we conclude that TNP 18, TNP 30, and TNP 87 induced a dose- and size-dependent cytotoxicity via decreased cell viability, increased LDH and ROS levels by in vitro methods.

Graphical Abstract

Keywords:

• Nano titanium
• TEM characterization
• cytotoxicity
• IC50
• ROS release

Acknowledgments

Authors thank the NCCS, Pune, India for supply of cell lines for this study.

Disclosure statement

The authors report that there are no conflicts of interest.

Additional information

Funding

The first author was grateful to the University Grants Commission (UGC), New Delhi, India, for support of funding in the form National Fellowship.