The effects of titanium nanoparticles on enzymatic and non-enzymatic biomarkers in female Wistar rats

Abstract

Titanium dioxide (TiO₂) nanoparticles (NPs) are widely used in industry, pharmacy, medicine, and food sectors. Therefore, this study deals with the effects of TiO₂ NPs in female rats following oral administration in differing doses for 14 days (0, 0.5, 5, and 50 mg/kg b.w./d). The response of enzymatic biomarkers (Na,K-ATPase, Mg-ATPase, and AChE) was measured in the brain, kidney, and small intestine, while non-enzymatic biomarker levels, such as different forms of glutathione (GSH) and thiobarbituric acid reactive substances (TBARSs) were measured in the liver. The images of the tissues were obtained using a transmission electron microscope (TEM) to demonstrate TiO₂ NP accumulation. Data showed that brain AChE activity decreased at all TiO₂ NP doses, though brain ATPase activities increased. However, ATPase activities in the intestine and kidney did not change significantly. Levels of GSH forms did not change significantly, though there was a significant decrease in TBARS level at the highest NP dose. TEM images demonstrated that TiO₂ NPs accumulated in a dose-dependent manner in the tissues. Data emphasized that the brain was the most sensitive organ against the effects of TiO₂ NPs. This study suggests the need for further studies to evaluate better the toxic effects of TiO₂ NPs.

Keywords:

• Metal
• titanium
• nanoparticle
• rat
• biomarker
• TEM

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The Research Fund of Cukurova University partly supported this study (FDK-2017-8197).