Investigation of the role of K_ATP channels in the cytotoxic effect of cypermethrin on rat-derived aortic smooth muscle cells

Abstract

We investigate role of ATP sensitive potassium (K_ATP) channel in cytotoxic effect of cypermethrin on rat aortic smooth muscle cells. Cytotoxicity analysis was performed at 0, 0.1, 0.5, 10, 50, and 100 µM concentrations of cypermethrin and the cell index (CI) was calculated. K_ATP currents were recorded using patch clamp technique for 50 and 100 µM concentrations and channel conductivity was determined by obtaining current-voltage characteristics. No cytotoxic effect was observed in the first 72 hours. At the 96th hour, only at 100 µM concentration, the CI value decreased significantly compared to control group and at 120 and 144th hours, it was observed that the CI value decreased significantly at all concentrations. Currents and conductivities were significantly decreased at 50 and 100 µM concentrations. Results gave clues that cypermethrin causes a cytotoxic effect on vascular smooth muscles and that K_ATP channels may have a role in the emergence of this effect.

Keywords:

• Cypermethrin
• xCELLigence
• cell index
• K_ATP channels
• patch clamp
• aortic smooth muscle

Acknowledgement

We would like to thank Professor Ş. Necat YILMAZ for his support and contributions during this study.

Author contributions

FS and ÜÇ designed the study; ÜÇ drafted the manuscript; FS, CU, and DK assisted in writing; FS and ÜÇ carried out the analysis and interpreted the results; FS, CU and DK were responsible for the data collection; all authors reviewed the final manuscript and gave their consent.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article.

Additional information

Funding

This study was supported by Mersin University with the project number 2016–2-AP1-1948.