Salvia officinalis attenuates bleomycin-induced oxidative stress and lung fibrosis in rats

Abstract

Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by progressive and irreversible fibroblasts proliferation leading to significant respiratory insufficiency. This study was designed to investigate the effect of sage infusion against bleomycin (BLM)-induced lung fibrosis in rats. Male Wistar rats were given a single dose of BLM (4 mg/kg, intratracheal), while sage infusion (50, 100 and 150 mg/kg, intraperitoneal) was administered 3 day later and continued for 4 weeks. We reveled by HPLC and LC–MS methods an important amount of phenolic bioactive compounds such as vanillic, gallic, ellagic, rosmarinic and carnosic acids. BLM induced collagen deposition, increased lipid peroxidation (MDA) and decreased superoxide dismutase (SOD) and catalase (CAT) activities. Only sage infusion at 150 mg/kg normalized MDA and antioxidant enzyme levels (SOD and CAT) and reduced significantly lung fibrosis. Our results showed also that this high dose have no renal or hepatic cytotoxic effect. In conclusion, sage can protect against BLM-induced murine lung fibrosis and oxidative stress due to the large content of bioactive phenolic compounds.

Keywords:

• Salvia officinalis;
• lung fibrosis;
• oxidative stress;
• bioactive compounds

Additional information

Funding

This scientific work was funded by the Tunisian Ministry of High Education and Scientific Research.

Notes on contributors

Sana Bahri

SB, RBA and SJ conceived and designed the research. SB, RBA, AF, RA and MM conducted experiments. SB and SJ analyzed data. SB wrote the manuscript. SJ revised the manuscript. All authors read and approved the manuscript.

Ridha Ben Ali

SB, RBA and SJ conceived and designed the research. SB, RBA, AF, RA and MM conducted experiments. SB and SJ analyzed data. SB wrote the manuscript. SJ revised the manuscript. All authors read and approved the manuscript.

Afef Nahdi

SB, RBA and SJ conceived and designed the research. SB, RBA, AF, RA and MM conducted experiments. SB and SJ analyzed data. SB wrote the manuscript. SJ revised the manuscript. All authors read and approved the manuscript.

Mona Mlika

SB, RBA and SJ conceived and designed the research. SB, RBA, AF, RA and MM conducted experiments. SB and SJ analyzed data. SB wrote the manuscript. SJ revised the manuscript. All authors read and approved the manuscript.

Raed Abdennabi

SB, RBA and SJ conceived and designed the research. SB, RBA, AF, RA and MM conducted experiments. SB and SJ analyzed data. SB wrote the manuscript. SJ revised the manuscript. All authors read and approved the manuscript.

Saloua Jameleddine

SB, RBA and SJ conceived and designed the research. SB, RBA, AF, RA and MM conducted experiments. SB and SJ analyzed data. SB wrote the manuscript. SJ revised the manuscript. All authors read and approved the manuscript.