Advanced search
Publication Cover
Journal of Neurogenetics Volume 19, 2005 - Issue 3-4
Submit an article Journal homepage
378
Views
22
CrossRef citations to date
0
Altmetric
Original

TARGETED EXPRESSION OF IP3 SPONGE AND IP3DSRNA IMPAIRES SUGAR TASTE SENSATION IN DROSOPHILA

Kazue Usui-Aoki Waseda University, School of Science and Engineering, Nishi-Tokyo, Japan; Current address: Chiba industry advancement center, JST-CREATE, Kisarazu, Japan
,
Ken Matsumoto Waseda University, School of Science and Engineering, Nishi-Tokyo, Japan
,
Masayuki Koganezawa Tohoku University, Graduate School of Life Sciences, Sendai, Japan
,
Sou Kohatsu Tohoku University, Graduate School of Information Sciences, Sendai, Japan
,
Kunio Isono Tohoku University, Graduate School of Information Sciences, Sendai, Japan
,
Hiroshi Matsubayashi Kyoto Institute of Technology, Drosophila Genetic Resource Center, Kyoto, Japan
,
Masa-Toshi Yamamoto Kyoto Institute of Technology, Drosophila Genetic Resource Center, Kyoto, Japan
,
Ryu Ueda National Institute of Genetics, Mishima, Japan
,
Kuniaki Takahashi National Institute of Genetics, Mishima, Japan
,
Kaoru Saigo The University of Tokyo School of Science, Tokyo, Japan
,
Katsuhiko Mikoshiba The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
&
Daisuke Yamamoto Waseda University, School of Science and Engineering, Nishi-Tokyo, Japan; Tohoku University, Graduate School of Life Sciences, Sendai, JapanCorrespondence[email protected]
 show all
Pages 123-141 | Received 26 Aug 2005, Accepted 29 Dec 2005, Published online: 11 Jul 2009
 
Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days

Abstract

We evaluated the role of IP3 in sugar taste reception in Drosophila melanogaster by inactivating the IP3 signaling using genetic tools. We used the “IP3 sponge,” composed of the modified ligand-binding domain from the mouse IP3 receptor, which was designed to absorb IP3 in competition with native IP3 receptors. Another tool was a transgene that generates double-stranded RNA against IP3 receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP3 signaling is indispensable for sugar reception in Drosophila.

Log in via your institution

Access through your institution

Log in to Taylor & Francis Online

Restore content access

Restore content access for purchases made as guest
Purchase options * Save for later

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 65.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 1,079.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.