The desaturase1 gene affects reproduction before, during and after copulation in Drosophila melanogaster

Abstract

Desaturase1 (desat1) is one of the few genes known to be involved in the two complementary aspects of sensory communication — signal emission and signal reception — in Drosophila melanogaster. In particular, desat1 is necessary for the biosynthesis of major cuticular pheromones in both males and females. It is also involved in the male ability to discriminate sex pheromones. Each of these two sensory communication aspects depends on distinct desat1 putative regulatory regions. Here, we used (i) mutant alleles resulting from the insertion/excision of a transposable genomic element inserted in a desat1 regulatory region, and (ii) transgenics made with desat1 regulatory regions used to target desat1 RNAi. These genetic variants were used to study several reproduction-related phenotypes. In particular, we compared the fecundity of various mutant and transgenic desat1 females with regard to the developmental fate of their progeny. We also compared the mating performance in pairs of flies with altered desat1 expression in various desat1-expressing tissues together with their inability to disengage at the end of copulation. Moreover, we investigated the developmental origin of altered sex pheromone discrimination in male flies. We attempted to map some of the tissues involved in these reproduction-related phenotypes. Given that desat1 is expressed in many brain neurons and in non-neuronal tissues required for varied aspects of reproduction, our data suggest that the regulation of this gene has evolved to allow the optimal reproduction and a successful adaptation to varied environments in this cosmopolitan species.

Keywords:

• Evolution
• sexual isolation
• cuticular hydrocarbon
• MARCM
• mcAL neurons
• mAL neurons

Acknowledgments

We thank Dr. Hiromu Tanimoto and all colleagues from his lab for organizing the symposium dedicated for the work of Professor Daisuke Yamamoto, two anonymous reviewers for in-depth reading of the first version of this ms, and Jérôme Cortot, Serge Loquin and José Solonot for technical help.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was partially funded by the Center National de la Recherche Scientifique (Insb), by the Burgundy Regional Council (PARI2010, PARI2012) and by the ANR (INSAVEL).