Effect of latex material on antioxidant enzymes, lipid peroxidation, DNA damage, and chromosomal aberration

Abstract

Cell integrity is affected by oxidative stress when the production of active oxidants overwhelms antioxidant defense mechanisms. Latex, a natural polymer obtained from Hevea brasiliensis, is used in medical industry for manufacturing surgical gloves, urinary catheters, and dental dams. The aim of this study was to evaluate the effects of latex material on oxidative stress by in vivo and in vitro methods. In addition, the material was screened for its ability to induce any chromosomal aberrations (CAs) by in vitro method. In vivo studies were carried out with implanted latex material onto subcutaneous tissue of various batches of experimental Wistar rats. At the end of experimental period, animals were anesthetized, blood was collected for serum analysis, and sacrificed. Liver was excised for the determination of antioxidant enzymes and lipid peroxidation (LPO). Subcutaneous tissues were obtained for the extraction of genomic DNA from implanted animals and checked for the presence of 8-hydroxy-2-deoxyguanosine (8-OHdG), considered an indicator of DNA damage. Simultaneously, in vitro studies were carried out using fresh liver and subcutaneous tissue obtained from Swiss albino mice treated with physiological saline extract of latex material. For the estimation of both in vitro and in vivo oxidative stress, 10% liver homogenate was assessed for stress indicators like reduced glutathione, glutathione reductase, glutathione peroxidase, LPO and protein content. The results of both in vivo and in vitro studies indicated that the chemical leachents from the latex material did not significantly affect LPO and the levels of antioxidant enzymes. There was also no significant increase in 8-OHdG content due to the presence of implanted latex material. Finally, the results of in vitro CA test and G banding indicated that extracts of test material did not induce any chromosomal abnormalities.

Keywords:

• reactive oxygen species
• lipid peroxidation
• reduced glutathione
• glutathione reductase
• glutathione peroxidase
• chromosomal aberration
• 8-OHdG

Acknowledgments

Authors are thankful to the Director and Head, SCTIMST, for providing the facilities to carry out the work. We are also thankful to Dr Ramesh, Polymer Processing Laboratory, SCTIMST, for providing us with the material. Authors also acknowledge Shaji S and Harikumar for their technical assistance. The work was suFpported by the grants from Indian Council of Medical Research (ICMR) (Grant No.5/20/1 (Bio)/07-NCD-I), New Delhi.

Notes

Ames

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