Ameliorative effect Trichosanthes dioica root against arsenic-induced brain toxicity in albino rats

Abstract

This study evaluated the ameliorative potential of hydroalcoholic extract of Trichosanthes dioica root (TDA) against arsenic (As)-induced toxicity in rat brain. TDA (5 and 10 mg kg⁻¹) was administered orally to Wistar albino rats for 20 consecutive days before oral administration of sodium arsenite (10 mg kg⁻¹) for 8 days. Brain biochemical parameters including lipid peroxidation, reduced and oxidized glutathione, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase, and DNA fragmentation were determined. Pretreatment with TDA significantly and dose-dependently modulated all the antioxidative parameters and reduced DNA fragmentation in As-exposed rat brain. Data indicate that T. dioica root produced remarkable ameliorative effects against As-induced brain toxicity in rats mediated by modulation of oxidative stress.

Keywords:

• arsenic toxicity
• Trichosanthes dioica
• root
• brain
• oxidative stress
• sodium arsenite

Acknowledgments

The authors are thankful to the authorities of Bengal School of Technology, Sugandha, Hooghly 712102, India, and Jadavpur University, Kolkata 700032, India, for providing necessary facilities for this study. The authors are also grateful to the All India Council of Technical Education (AICTE), New Delhi, India, for providing technical supports for this study. Conflict of interest: The authors declare that there is no conflict of interest.