Chromosome 3p Microsatellite Allelotyping in Neuroblastoma: A Report on the Technical Hurdles

Abstract

Pinpointing critical regions of recurrent loss may help localize tumor suppressor genes. To determine the regions of loss on chromosome 3p in neuroblastoma, we performed loss of heterozygosity analysis using 16 microsatellite markers in a series of 65 primary tumors and 29 neuroblastoma cell lines. In this study, we report the results and discuss the technical hurdles that we encountered during data generation and interpretation that are of relevance for current studies or tests employing microsatellites. To provide functional support for the implication of 3p tumor suppressor genes in this childhood malignancy, we performed a microcell-mediated chromosome 3 transfer in neuroblastoma cells.

Keywords:

• Loss of heterozygosity
• Chromosome 3p
• Neuroblastoma
• Microsatellite
• Microcell-mediated chromosome transfer