Microbial interactions during sugar cane must fermentation for bioethanol production: does quorum sensing play a role?

Abstract

Microbial interactions represent important modulatory role in the dynamics of biological processes. During bioethanol production from sugar cane must, the presence of lactic acid bacteria (LAB) and wild yeasts is inevitable as they originate from the raw material and industrial environment. Increasing the concentration of ethanol, organic acids, and other extracellular metabolites in the fermentation must are revealed as wise strategies for survival by certain microorganisms. Despite this, the co-existence of LAB and yeasts in the fermentation vat and production of compounds such as organic acids and other extracellular metabolites result in reduction in the final yield of the bioethanol production process. In addition to the competition for nutrients, reduction of cellular viability of yeast strain responsible for fermentation, flocculation, biofilm formation, and changes in cell morphology are listed as important factors for reductions in productivity. Although these consequences are scientifically well established, there is still a gap about the physiological and molecular mechanisms governing these interactions. This review aims to discuss the potential occurrence of quorum sensing mechanisms between bacteria (mainly LAB) and yeasts and to highlight how the understanding of such mechanisms can result in very relevant and useful tools to benefit the biofuels industry and other sectors of biotechnology in which bacteria and yeast may co-exist in fermentation processes.

Keywords:

• Bioethanol
• yeast
• lactic acid bacteria
• microbial interactions
• quorum sensing

Acknowledgements

The authors acknowledge the financial support of 'Conselho Nacional de Desenvolvimento Científico e Tecnológico' (CNPq) (Grant #302763/2014–7) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the financial support of this project.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors acknowledge the financial support of 'Conselho Nacional de Desenvolvimento Científico e Tecnológico' (CNPq) (Grant #302763/2014–7) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the financial support of this project.