Antioxidant, anti-quorum sensing, biofilm inhibitory activities and chemical composition of Patchouli essential oil: in vitro and in silico approach

Abstract

The interest in naturally occurring essential oils from medicinal plants has increased extremely over the last decade markedly because they possess antimicrobial and antioxidant protective properties against different chronic diseases. Extensive survival of drug-resistant infectious bacteria depends on quorum sensing (QS) signaling network which raises the need for alternative antibacterial compounds. The aim of this study was to examine the phytochemical compounds of patchouli essential oil (PEO) and to assess its antioxidant activity. Antioxidant studies estimated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method showed that the PEO has effective antioxidant activity (IC₅₀ 19.53 µg/mL). QS inhibitory activity of PEO was examined by employing the biosensor strain, Chromobacterium violaceum CV12472. At sub-lethal concentrations, PEO potentially reduced the QS regulated violacein synthesis in CV12472 without inhibiting its cell proliferation. Moreover, it also effectively reduced the production of some QS regulated virulence factors and biofilm development in P. aeruginosa PAO1 without hindering its growth. Phytochemical analysis of PEO was done by GC/MS technique. Molecular docking of PEO major compounds with QS (LasR and FabI) and biofilm regulator proteins (MvfR and Sialidase) of PAO1 was evaluated. These phytocompounds showed potential hydrogen binding interactions with these proteins. The overall results, in vitro and in silico, suggest that PEO could be applied as biocontrol agent against antibiotic resistance pathogens.

Communicated by Ramaswamy H. Sarma

Keywords:

• Pogostemon cablin
• Patchouli essential oil
• anti-oxidant
• quorum sensing inhibition
• anti-biofilm
• GC-MS analysis
• molecular docking

Acknowledgements

Authors thanks, Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr D. Y. Patil Vidyapeeth, Tathawade, Pune, for providing necessary facilities. Kiran Bharat Lokhande acknowledges the Indian Council of Medical Research (ICMR), New Delhi, for Senior Research Fellowship (Project ID: 2019-3458; File: ISRM/11(54)/2019).

Disclosure statement

The authors have declared that no conflict of interest exists.

Additional information

Funding

This work received funding from Dr. D. Y. Patil Vidyapeeth, Pune [DPU/752(7)/2017 and DPU/421/2018].