Abstract
In this report, synthesis, characterization, biological and molecular modeling studies of Nophen Schiff base [N,N-bis(2-hydroxy-1-naphthaldehyde)-o-phenylenediamine] and Co(II)-Nophen complex have been furnished. BSA binding affinities of the ligand and Co(II)-Nophen complex have been appraised by UV-visible, fluorescence and cyclic voltammetric techniques. Spectroscopic measurements indicate strong binding of the complex with BSA protein through static quenching mechanism with binding constant in the order of 104 M−1. The negative shift of the peak potential in cyclic voltammetry suggested an electrostatic interaction. Molecular docking analysis reveals significant binding affinity (-6.3 kcal/mol) of the complex towards BSA protein. It is amazing that the in vitro cytotoxicity of Co(II)-Nophen complex against A549 cell lines (Human lung carcinoma cells) has remarkable potentials with 29 ± 1.2 µM as IC50 value. Comparing the biological activity towards microorganisms, Co(II)-Nophen complex show substantial response than the Nophen ligand.
Communicated by Ramaswamy H. Sarma
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Highlights
Synthesis and Characterization of Co(II)-Nophen complex derived from Nophen ligand
Analytical, computational and spectral techniques are performed
BSA binding and molecular modeling of ligand and Co(II)-Nophen complex are studied
Antimicrobial activity and cytotoxicity of synthesized compounds are examined
In vitro cytotoxicity against A549 cell lines is notable for Co(II)-Nophen complex
Disclosure statement
The authors declare no competing interests.