Diet-induced obesity reduces core body temperature across the estrous cycle and pregnancy in the rat

ABSTRACT

Obesity during pregnancy causes adverse maternal and fetal health outcomes and programs offspring for adult-onset diseases, including cardiovascular disease. Obesity also disrupts core body temperature (T_c) regulation in nonpregnant rodents; however, it is unknown whether obesity alters normal maternal T_c adaptations to pregnancy. Since T_c is influenced by the circadian system, and both obesity and pregnancy alter circadian biology, it was hypothesized that obesity disrupts the normal rhythmic patterns of T_c before and during gestation. Obesity was induced by cafeteria (CAF) feeding in female Wistar rats for 8 weeks prior to and during gestation, whereas control (CON) animals had free access to chow. Intraperitoneal temperature loggers measured daily T_c profiles throughout the study, while maternal body composition and leptin levels were assessed near term. Daily temperature profiles were examined for rhythmic features (mesor, amplitude and acrophase) by cosine regression analysis. CAF animals exhibited increased fat mass (93%) and associated hyperleptinemia (3.2-fold increase) compared to CON animals. CAF consumption reduced the average T_c (by up to 0.29°C) across the estrous cycle and most of pregnancy; however, T_c for CAF and CON animals converged toward the end of gestation. Obesity reduced the amplitude of T_c rhythms at estrus and proestrus and on day 8 of pregnancy, but increased the amplitude at day 20 of pregnancy. Photoperiod analysis revealed that obesity reduced T_c exclusively in the light period during pre-pregnancy but only during the dark period in late gestation. In conclusion, obesity alters rhythmic T_c profiles and reduces the magnitude of the T_c decline late in rat gestation, which may have implications for maternal health and fetal development.

KEYWORDS:

• Circadian
• core body temperature
• obesity
• pregnancy
• thermoregulation

Declaration of interest

The authors declare no conflict of interest. This research did not receive any specific funding in the public, commercial, or not-for-profit sector.