Streptococcus salivarius K12 inhibits Candida albicans aggregation, biofilm formation and dimorphism

Abstract

Candida albicans causes candidiasis, particularly in immunocompromised patients. Streptococcus salivarius K12 (K12) is a probiotic isolated from a healthy oral cavity. The study aimed to determine the effect of K12 on C. albicans aggregation, biofilm formation and dimorphism. C. albicans ATCC MYA-4901, acquired immunodeficiency syndrome (AIDS) isolate (ALC2), and oral cancer isolate (ALC3) and K12 were used in the study. All C. albicans strains and K12 were grown in yeast peptone dextrose agar and brain heart infusion agar, respectively, prior to aggregation, biofilm and dimorphism assays. Auto-aggregation of C. albicans MYA-4901 and ALC2 was categorised as high, while the co-aggregation of the strains was low in the presence of K12. C. albicans total cell count decreased significantly when co-cultured with K12 compared with monocultured C. albicans biofilm (p < 0.05). Inhibition of yeast-to-hyphae transition was also observed when co-cultured with K12. In conclusion, K12 inhibits C. albicans aggregation, biofilm formation and dimorphism.

Keywords:

• Polymicrobial interactions
• aggregation
• biofilm
• dimorphism
• probiotic

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This study was funded by the Ministry of Higher Education, Malaysia, for the funding (FRGS/1/2018/SKK11/UIAM/03/1), the International Islamic University Malaysia (IIUM) for providing the laboratory and the Melbourne Dental School, The University of Melbourne for the microbial samples.