Inertial Particle Deposition in a Monkey Nasal Mold Compared with that in Human Nasal Replicas

Abstract

Information on nasal particle deposition is used in risk assessments for exposure to airborne particulate pollutants and for optimizing the delivery of therapeutic aerosols. Monkeys are commonly used to assess the therapeutic potential of inhaled substances and to a lesser extent the toxicity of inhaled xenobiotics. Yet no reliable measurements of the deposition efficiency of monkey nasal airways for particles >1 μm have been reported to date. The goals of this study were to measure the deposition efficiency (>1 μm) of a replica of monkey nasal airways and to investigate potential differences in nasal deposition between humans and monkeys by comparing results with similar measurements recently reported for human nasal replicas. The monkey nasal replica was an acrylic mold made from a postmortem cast of the nasal airways of a 12-kg, male rhesus monkey. Particle deposition in the monkey nasal mold was measured for monodisperse aerosols between 1 and 10 μm and constant inspiratory flow rates between 2 and 7 lpm. Total deposition efficiency increased from nearly 0 to 100% with increasing particle inertia and was uniquely determined by values of an inertial impaction parameter. The deposition efficiencies of the monkey replica agreed well with those of human nasal replicas when compared according to equivalent Stokes numbers based on minimum cross-sectional area. Results from this study could improve monkey-to-human extrapolation models and interpretations of data from particle toxicity and therapeutic aerosol studies using monkeys.