Combined effects of artificial dermis and vascular endothelial growth factor concentration gradient on wound healing in diabetic porcine model

Abstract

Wounds in patients with diabetes mellitus are one of the most prevalent impaired wounds in the world. Vascular endothelial growth factor (VEGF) is one of the most important proangiogenic mediators. Artificial dermal (AD) such as Pelnac^® has been shown, in humans and animal models, a great therapeutic potential in full-thickness skin wounds. We attempt to promote the wound healing in diabetic porcine models through combined use of AD and constant concentration of VEGF or VEGF concentration gradient. We created full-thickness excisional wounds in diabetic animal models. Analyzed the healing process through images, histology and immunohistochemistry. Results show that the combination of AD and concentration gradient of VEGF could provide an appropriate angiogenesis, improve granulation formation, increase epithelization and maintain the VEGF levels of the wound bed. Eventually accelerate the direct healing of diabetic wounds or make good preparation for secondary skin graft.

Keywords:

• VEGF
• concentration gradient
• artificial dermal
• diabetic wound
• wound healing

Acknowledgements

This study was supported by the National Nature Science Foundation of China [Grants Nos. 81272108 and 81671922], Key Project of Nanjing Bureau of Health [ZKX13024], Project of Nanjing Science and Technology Development [201201069].

Disclosure statement

The authors have no conflicts of interest or financial ties to declare.

Additional information

Funding

This study was supported by the National Nature Science Foundation of China [Grants Nos. 81272108 and 81671922], Key Project of Nanjing Bureau of Health [ZKX13024], Project of Nanjing Science and Technology Development [201201069].