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Research Article

A novel procedure for preparation of submicron liposomes-lyophilization of oil-in-water emulsions

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Pages 231-240 | Received 06 Nov 2008, Accepted 31 Jan 2009, Published online: 21 Aug 2009
 

Abstract

A novel liposome preparation method is described involving the freeze-drying of oil-in-water emulsions containing sucrose in the aqueous phase as a lyoprotectant and phospholipids in the oil as emulsifiers. The oil-in-water emulsions can be prepared by emulsification and then lyophilized to obtain dry products that will, upon rehydration, form unilamellar liposomes with a mean size of less than 200 nm, as proved by dynamic light scattering, atomic force micrographs, freeze-fracture electron micrographs, and small-angle X-ray scattering (SAXS). With or without combination with active loading, this method can be used successfully to encapsulate amphiphilic or lipophilic drugs into liposomes, although it cannot be applied to hydrophilic drugs. The lyophilized products are stable and can be rehydrated to form liposomes, even after a storage period of 10 months. Based on the encapsulation efficiency for hydrophilic drugs, as well as the scanning electron micrograph and SAXS of the freeze-dried products, a unique liposome formation mechanism is proposed.

Acknowledgments

This work was partly supported by S&R Project-Starting Off Funds of JMC. We are grateful to Dr. Jinrong Liu for obtaining the atomic force micrographs. The authors would also like to thank Li Zheng from the central laboratory of SPU for her assistance in the experimental work.

Declaration of interest: The authors report no financial conflicts of interest. The authors alone are responsible for the content and writing of this paper.

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