Association of novel polymorphisms in caprine SOD3 gene with physiological and biochemical parameters

ABSTRACT

The foremost reaction of animals under thermal stress is increase in respiration rate, rectal temperature and heart rate which directly affects feed intake thereby, reduces growth rate, milk yield, reproductive performance, and even death in extreme cases. In the present study polymorphism in SOD3 gene was identified and their relation with physiological vital parameters like rectal temperature, skin temperature, heart rate, pulse rate and respiration rate alongwith biochemical parameters like serum levels of albumin, bilirubin, total proteins, creatinine, urea, total cholesterol, high density lipoprotein (HDL) cholesterol, triglycerides, asparate aminotransferase (AST) and ALT was estimated during heat stress. Temperature-humidity index (THI) was calculated for different regions and statistical analysis was performed to find out any association of THI with physiological vital parameters and biochemical parameters. The higher value of physiological vital parameters indicated that heat stress may be the most probable cause for such significant difference. The haplotypes were identified after PCR amplification and sequencing of the SOD3 gene. The CGC and CAA haplotypes showed lower values of physiological vital parameters making these haplotypes to be more heat tolerant than others. The study of biochemical parameters revealed the similar results establishing superior heat tolerance capacity in CGC and CAA haplotypes.

KEYWORDS:

• Gene
• goat
• haplotype
• SOD3
• stress

Acknowledgments

The authors are thankful to the authority of Orissa University of Agriculture and Technology for providing the necessary facility to carry out the research work.

Disclosure statement

No potential conflict of interest was reported by the authors.

Compliance with ethical standards

We testify on behalf of all coauthors that our article entitled “Association of SOD3 gene polymorphism with heat tolerance traits in goats” has not been published in whole or in part elsewhere; the manuscript is not currently being considered for publication in another journal; all authors have been personally and actively involved in substantive work leading to the manuscript and will hold themselves jointly and individually responsible for its content.

Additional information

Funding

The necessary funding for the research work was provided by Indian Council of Agricultural Research, New Delhi, India (ICAR Extramural Project).