Effect of irisin on endometrial receptivity of rats with polycystic ovary syndrome

Abstract

To investigate the influence of irisin on endometrial receptivity of rats with polycystic ovarian syndrome (PCOS). PCOS rats were randomly divided into PCOS group and irisin group, and normal rats were used as control group. The PCOS group and control group were injected intraperitoneally with normal saline while the irisin group with recombinant irisin. The serum and uterus were obtained. Detect serum sex hormones, including Testosterone (T), Estradiol (E₂), Progesterone (P), and glucose, insulin levels. Observe endometrial morphology by hematoxylin-eosin staining. Then evaluate the expression of leukemia inhibitory factor (LIF) and integrin αvβ3 in endometrium using ELISA, immunohistochemistry and Real-time PCR. (1) Levels of serum T, glucose and insulin in PCOS group were significantly higher than those in control and irisin group. (2) For the endometrial morphology, levels of equivalent diameter, area of uterine glands and gland cavity and endometrial average thickness were lower in PCOS group than those in control and irisin group. (3) LIF and integrin αvβ3 mRNA were basically consistent with protein expression. Levels of LIF and integrin αvβ3 were decreased in PCOS group when compared with control and irisin group. Irisin may improve endometrial receptivity by promoting expression of LIF and integrin αvβ3.

摘要

本文是为探讨鸢尾素对多囊卵巢综合征（PCOS）大鼠子宫内膜容受性的影响。PCOS大鼠被随机分为PCOS组和鸢尾素组, 对照组为正常大鼠。PCOS组和对照组给予腹腔注射生理盐水, 鸢尾素组给予腹腔注射重组鸢尾素。获得血清及大鼠的子宫进行检测。检测血清性激素, 包括睾酮（T）、雌二醇（E2）、孕酮（P）、葡萄糖和胰岛素水平。通过苏木精-伊红染色观察子宫内膜形态。用酶联免疫吸附试验、免疫组化和实时聚合酶链反应（PCR）检测白血病抑制因子（LIF）和整合素αvβ3在子宫内膜的表达。（1）PCOS组血清T、葡萄糖、胰岛素水平明显高于对照组和鸢尾素组。（2）在子宫内膜形态上, 多囊卵巢综合征组的等效直径、子宫腺面积、腺腔面积和子宫内膜平均厚度均低于对照组和鸢尾素组。（3）LIF和整合素αvβ3mRNA的表达与蛋白表达基本一致。多囊卵巢综合征组的LIF和整合素αvβ3水平与对照组和鸢尾素组相比较有所下降。鸢尾素可通过促进LIF和整合素αvβ3的表达来改善子宫内膜容受性。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Irisin
• polycystic ovarian syndrome
• leukemia inhibitory factor
• integrin αvβ3

Disclosure statement

No potential conflict of interest was reported by the authors.