Positive effect of a new supplementation of vitamin D₃ with myo-inositol, folic acid and melatonin on IVF outcomes: a prospective randomized and controlled pilot study

Abstract

To assess whether oral supplementation of vitamin D₃, myo-inositol, folic acid and melatonin affects IVF outcomes. One hundred and twenty consecutive infertile women attending IVF treatment were 1:1 randomly distributed in two groups. Women in group A (control) were assigned to receive myo-inositol, alpha-lactalbumin and folic acid in the morning, and myo-inositol, folic acid and melatonin in the evening. Women in group B (treated) were assigned to receive analogous treatment, with the addition of cholecalciferol (vitamin D₃) in the evening from the early beginning of the luteal phase. 50 patients in group A and 50 in group B underwent blastocyst transfer and were considered in the statistical analysis. Vitamin D₃ levels significantly increased after 45 days of treatment: 33.2 ng/ml in group B Vs. 24.3 ng/ml in group A (p < .0001). The implantation rate increased as well: 37.1% in group B Vs. 19.2% in group A (p < .0151). Overall, the results indicate that increased vitamin D₃ levels positively correlate with the implantation rate in IVF. Because of the low number of participants, these findings need to be confirmed with larger cohorts of patients.

摘要

评估口服维生素D₃、肌醇、叶酸和褪黑素是否影响IVF结局。120例连续接受IVF治疗的不孕女性按1：1随机分为两组。A组（对照组）早上口服肌醇、α-乳白蛋白和叶酸, 同时夜间补充肌醇、叶酸和褪黑素。B组（治疗组）的女性接受类似的治疗, 且从黄体期早期开始夜间加用胆钙化醇（维生素D₃）。经历囊胚移植的A组50例, B组50例女性均纳入统计分析, 治疗45天后维生素D₃水平显著升高：B组为33.2 ng/ml, A组24.3 ng/ml（p<0.0001）。着床率也增加：B组着床率为37.1%, 明显高于A组19.2%（p<0.0151）。总体而言, 研究结果表明, 维生素D₃水平的升高与IVF的着床率呈正相关。由于参与者数量较少, 这些发现需要在更大的患者队列中得到证实。

Keywords:

• Vitamin D3
• IVF
• ICSI
• implantation rate
• endometrial receptivity

Disclosure statement

No potential conflict of interest was reported by the author(s).