Evaluation of thiol/disulfide homeostasis in patients with gestational diabetes mellitus

Abstract

This study aimed to study the dynamic thiol/disulfide homeostasis in pregnant women with gestational diabetes mellitus (GDM). Forty-five pregnant women with GDM and 45 age-matched healthy pregnancies were included in this study. Thiol/disulfide homeostasis was measured using a commercial kit (Rel Assay Diagnostics). The patients with GDM had significantly higher disulfide concentrations than healthy pregnant patients (p = .001). Besides, the GDM group had significantly higher disulfide/total thiol, disüplhide/native thiol, and native thiol/total thiol ratio than healthy pregnant patients (p = .001, p = .001 and p = .001, respectively). The significantly higher concentrations of disulfide, disulfide/total thiol, disüplhide/native thiol, and native thiol/total thiol ratio in women with GDM could be considered as the increased oxidative stress.

摘要

本研究旨在研究妊娠期糖尿病(GDM)孕妇体内巯基/二硫代谢物的动态稳态。45名妊娠期糖尿病孕妇和45名年龄匹配的健康孕妇参加了这项研究。使用商业试剂盒(REL化验诊断)测量硫醇/二硫化物动态平衡。妊娠期糖尿病患者的二硫化物浓度显著高于健康孕妇(p＝.001)。此外, 妊娠期糖尿病组二硫/总硫醇、二硫/天然硫醇、天然硫醇/总硫醇比值均显著高于健康孕妇(分别为P＝.001、P＝.001和P＝.001)。妊娠期糖尿病患者体内二硫化物、二硫化物/总硫醇、二硫化氢/天然硫醇、天然硫醇/总硫醇比值明显升高, 可认为是氧化应激增加。

Keywords:

• Oxidative stress
• thiol/disulfide homeostasis
• gestational diabetes mellitus

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit, Project number: TSA-2019-3096.