miR-203a-3p, ABL1 and TP63 gene expression is altered in the endometrium of women with endometriosis

Abstract

Objective

Many genes and miRNAs have been shown to be associated with the pathogenesis of endometriosis. TP63 (p63) is implicated in lineage specification, proliferative potential, differentiation, cell death and survival. The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase implicated in cell differentiation, cell division, and cell adhesion. Moreover, hsa-miR-203a-3p was reported to play pivotal roles in tumor progression by targeting multiple genes, including ABL1 and TP63. The aim of this study was to investigate the expression of ABL1, TP63, and miR-203a-3p in endometriosis.

Methods

This study included 30 women with endometriosis (stage III: n = 12 and stage IV: n = 18) and 30 age-matched controls. Total RNA extraction and cDNA synthesis were performed, and a quantitative polymerase chain reaction technique was used to determine the expression of miR-203a-3p, TP63, and ABL1.

Results

TP63 and ABL1 were significantly overexpressed in stages III and IV endometriosis as compared to controls (p < .0001). Moreover, overexpression of ABL1 and TP63 was observed in stage IV compared to stage III (p = .0006 and p = .0002, respectively). Furthermore, significant increase miR-203a-3p expression has been seen in stage IV endometriosis compared to controls (p = .006). The expression of miR-203a-3p in stage III was not significant compared to stage IV and control (p = .33 and p = .43, respectively).

Conclusion

It is concluded that miR-203a-3p, ABL1 and TP63 gene expression is altered in the endometrium of patients with endometriosis. It is also suggested that miR-203a-3p, ABL1, and TP63 might be candidate factors for the pathogenesis of endometriosis and suggesting its therapeutic potential in endometriosis.

摘要

背景：应用雌激素和/或孕激素对增加乳腺增生（MGH）风险的效果存在争议。因此, 我们进行了荟萃分析, 以进一步阐明激素与MGH之间的关系。

方法：检索截至2021年4月28日前PubMed、Embase、WEB OF SCIENCE数据库、Cochrane图书馆、中国知识资源总库和万方数据库。

结果：本荟萃分析纳入9项相关研究。研究发现雌激素和/或孕激素的应用与MGH风险的增加有显著关联（RR = 1.56, 95%CI:1.13-2.15, p=.000）。亚组结果显示, 混合人群中MGH的风险增加（RR = 1.72, CI:1.58-1.88, p<.001）, 但在亚洲人群中无显著差异。同时, 对于使用雌孕激素联合应用（EPP）和绝经后女性, MGH的风险分别增加（RR = 1.74, CI:1.22-2.47, p=.002）和（RR = 1.75, CI:1.24-2.47, p=.001）, 但单独应用雌激素和绝经前女性没有显著差异。

结论：本研究结果表明, 应用雌激素和/或孕激素可能会增加绝经前和绝经后女性发生MGH的风险。

Keywords:

• Endometriosis
• miR-203a-3p
• ABL1
• TP63
• expression

关键词:

• #x06FC0;素
• 雌激素
• 孕激素
• 乳腺增生

Acknowledgments

This study was supported by the University of Guilan.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Funding

The author(s) reported there is no funding associated with the work featured in this article.