G protein-coupled estrogen receptor (GPER/GPR30) levels in pelvic floor muscles and its association with estrogen status in female rabbits

Abstract

Objective: To assess the relative expression of the G-protein coupled estrogen receptor (GPER) in the bulbospongiosus (Bsm) and pubococcygeus (Pcm) muscles in control, ovariectomized (OVX), and OVX with estradiol benzoate supplementation (OVX + EB) rabbits.Methods: We used tissues from C, 1-month OVX, and OVX plus 15-day EB implanted (OVX + EB) groups. The GPER expression was evaluated by Western blot and immunohistochemistry for both Bsm and Pcm. Results: Both muscles showed a GPER immunoreactivity in blood vessels, inside myofibers next to myonuclei, and in polymorphonuclear cells. Four-week ovariectomy did not modify the GPER expression in the Bsm and Pcm, but two-week estradiol benzoate increased it in the latter muscle alone.Conclusions: We demonstrated that the Bsm and Pcm of female rabbits express GPER. High serum estradiol levels elevate GPER relative expression in the Pcm alone. The present study supports the remarkable estrogen sensitivity of the Pcm.

摘要

目的

探讨G蛋白偶联雌激素受体(GPER)在正常母兔、去势母兔(OVX)和添加苯甲酸雌二醇的去势母兔(OVX+ EB)的兔球海绵肌(Bsm)和耻骨尾骨肌(Pcm)中的相对表达。方法:采用C组、OVX术后1个月组、OVX+ EB添加15天组。Western blot和免疫组化检测Bsm和Pcm中GPER的表达。

方法

采用C组、OVX术后1个月组、OVX+ EB添加15天组。Western blot和免疫组化检测Bsm和Pcm中GPER的表达。

结果

双侧肌肉血管、肌核旁肌纤维内及多形核细胞均有GPER免疫反应。卵巢切除4周没有改变Bsm和Pcm中GPER的表达, 但添加两周的雌二醇苯甲酸增加了Pcm中GPER的表达。

结论

雌性兔Bsm和Pcm表达GPER。高血清雌二醇水平可提高GPER在Pcm中的相对表达。本研究支持Pcm具有显著的雌激素敏感性。

Keywords:

• blood vessels
• GPER-1
• hormones
• micturition
• ovariectomy
• pelvic floor

关键词:

• 血管
• GPER-1
• 激素
• 排尿
• 卵巢切除术
• 盆底

Acknowledgments

The authors thank Laura García Rivera and Jesús Ramsés Chávez Ríos for the excellent technical assistance provided.

Data availability statement

The data supporting this study’s findings are available from the corresponding author, [FC], upon reasonable request.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México (DGAPA-UNAM) partially funded this study (PAPIIT IA203617 to FC). The Consejo Nacional de Ciencia y Tecnología of México provided pre-doctoral SYRJ (SYRJ, ID 781480) and masterdegree (GCHH, ID 861149) fellowships.