Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel

Abstract

The platelet transmembrane receptor GPVI can be assessed together with other platelet membrane markers in a whole blood multicolor flow cytometry panel. The advantage of combining multiple antibodies in a single tube is the possibility of distinguishing multiple platelet subgroups. In this short communication, we describe an activation problem encountered with anti-GPVI, clone HY101. Activation of platelets was seen after the addition of anti-GPVI in a flow cytometry panel, highlighted by the expression of the activation markers CD62P, PAC-1, CD63, and CD107a. This was also confirmed by platelet aggregation studies.

Keywords:

• Anti-GPVI
• flow cytometry
• HY101
• platelet

Author contributions

DB, MO, and MAVD performed the experiments for this original paper, which were further refined with SH and XS. MAVD did the writing of the paper with support of all other authors. All the authors approved the final version.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplementary material

Supplemental data for this article can be accessed on the publisher’s website.

Additional information

Funding

The author(s) reported that there is no funding associated with the work featured in this article.