Estimation of radiation tolerance to high LET heavy ions in an anhydrobiotic insect, Polypedilum vanderplanki

Abstract

Purpose: Anhydrobiotic larvae of Polypedilum vanderplanki are known to show an extremely high tolerance against a range of stresses. We have recently reported that this insect withstands exposure to high doses of gamma-rays (linear energy transfer [LET] 0.2 keV/μm). However, its tolerance against high LET radiation remains unknown. The aim of this study is to characterize the tolerance to high-LET radiations of P. vanderplanki.

Materials and methods: Larval survival and subsequent metamorphoses were compared between anhydrobiotic (dry) and non-anhydrobiotic (wet) samples after exposure to 1 – 7000 Gy of three types of heavy ions delivered from the azimuthally varying field (AVF) cyclotron with LET values ranging from 16.2 – 321 keV/μm. The tolerance against ⁴He ions was also compared among three chironomid species.

Results: At all LET values measured, dry larvae consistently showed greater radiation tolerance than hydrated larvae, perhaps due to the presence of high concentrations of the disaccharide trehalose in anhydrobiotic animals, and the radiation-induced damage became evident at lower doses as development progressed. Relative biological effectiveness (RBE) values based on the median inhibitory doses reached a maximum at 116 keV/μm (¹²C), and the maximum RBE clearly increased as development progressed. Lower D₀ (dose to reduce survival from relative value 1.00 – 0.37 on the exponential part of the survival curve), and higher D_q (quasi-threshold dose) were found in individuals exposed to ⁴He ions, compared to gamma-rays, and in P. vanderplanki larvae compared to non-anhydrobiotic chironomids.

Conclusion: Anhydrobiosis potentiates radiation tolerance in terms of larval survival, pupation and adult emergence of P. vanderplanki exposed to high-LET radiations as well as to low-LET radiation. P. vanderplanki larvae might have more efficient DNA damage repair after radiation than other chironomid species.

Keywords:

• Radiation tolerance
• high-LET radiation
• heavy ion
• anhydrobiosis
• Polypedilum vanderplanki
• trehalose