Abstract
Purpose: Effects on DNA damage response were investigated in murine L929 cells exposed to 50 Hz magnetic fields (MF) with or without ultraviolet B (UVB, wavelength 280–320 nm) radiation or menadione (MQ).
Materials and methods: Cells were exposed to MF at 100 or 300 μT combined with MQ (150 μM, 1 hour) or UVB radiation (160 J/m2) using various exposure schedules. The samples were stained with propidium iodide (PI) and analysed by flow cytometer for cell cycle stages. Apoptotic cells were defined as sub G1 events.
Results: In cells first exposed to 100 μT MF for 24 h, the response to subsequent MQ treatment was significantly altered so that the proportion of sub G1 cells was decreased and the proportion of cells in the G2/M phase was increased. When a 300 μT MF was used, also the proportion of cells in the G1 phase was decreased. MF exposures after MQ treatment did not alter responses to MQ. No effects were found from MF exposure alone or from MF combined with UVB radiation.
Conclusions: The results strengthen previous findings suggesting that pre-exposure to MF can alter cellular responses to other agents, and indicate that MF as low as 100 μT has measurable impacts on cancer-relevant cellular processes such as DNA-damage.