Proteomic analysis of irradiated lung tissue of mice using gel-based proteomic approach

Abstract

Purpose: Exposure to radiation causes severe alterations of protein expression level inside the cell, thus it may influence the biological events and stress response. In the present investigation, we have demonstrated the effect of radiation on mice lung tissues.

Materials and methods: Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF/TOF was used to check the expression changes in lung proteome profile of strain ‘A’ female mice after exposure to lethal doses of gamma irradiation at different time periods (24 and 48 h). Identified proteins were analysed for their altered expression and were further validated by Western blotting and enzyme-linked immunosorbent assay (ELISA).

Results: Nine significant differentially expressed proteins were identified from irradiated lungs tissues. The expression level of zinc finger protein was found to be up regulated at 24 h irradiation in comparison to 48 h irradiation.

Conclusions: Zinc finger protein may be considered as a radiation responsive protein. Alteration in its expression pattern may primarily affect binding specificity of the protein that can further result in the interference in transcriptional control of multiple stress responsive genes.

Keywords:

• Ionizing radiation
• lung proteome
• MALDI-TOF
• 2-DE
• zinc finger protein

Acknowledgements

We would like to acknowledge Defence Research & Development Organization (DRDO), Ministry of Defence, Indian Council of Medical Research (ICMR) and Council of Scientific and Industrial Research (CSIR) CARDIOMED BSC0122, Government of India, New Delhi, India, for providing financial support to carry out the research work.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Additional information

Funding

Defence Research & Development Organization, Ministry of Defence, Indian Council of Medical Research and Council of Scientific and Industrial Research.