Protective properties of Myrtus communis extract against oxidative effects of extremely low-frequency magnetic fields on rat plasma and hemoglobin

Abstract

Purpose: This study investigates the protective properties of Myrtus communis extract against the oxidative effects of extremely low-frequency magnetic fields (ELFMF). Also, this study is aimed to analyze the conformational changes of hemoglobin, oxidative damages to plasma proteins and antioxidant power of plasma following exposure to ELFMF.

Materials and methods: Adult male rats were divided into 3 groups: (1) control, (2) ELFMF exposure, and (3) ELFMF exposure after M. communis extract administration. The magnetic field (0.7 mT, 50 Hz) was produced by a Helmholtz coil for one month, 2 hours a day. The M. communis extract was injected intraperitoneally at a dose of 0.5 mg/kg before exposure to ELFMF. The oxidative effects of ELFMF were studied by evaluating the hemoglobin, methemoglobin (metHb) and hemichrome levels, absorption spectrum of hemoglobin (200–700 nm), oxidative damage to plasma proteins by measuring protein carbonyl (PCO) levels and plasma antioxidant power according to the ferric reducing ability of plasma (FRAP). The mean and standard errors of the mean were determined for each group. One-way ANOVA analysis was used to compare the means of groups. The significance level was considered to be p < .05. Moreover, artificial neural network (ANN) analysis was used to identify the predictive parameters for estimating the oxyhemoglobin (oxyHb) concentration.

Results: Exposure to ELFMF decreased the FRAP which was in concomitant with a significant increase in plasma PCO, metHb and hemichrome concentrations (p < .001). Oxidative modifications of Hb were shown by reduction in optical density at 340 nm (globin-heme interaction) and 420 nm (heme-heme interaction). Administration of M. communis extract increased FRAP values and decreased plasma POC, metHb, and hemichrome concentrations. Also, a significant increase in Hb absorbance at 340, 420, 542, and 577 nm showed the protective properties of M. communis extract against ELFMF-induced oxidative stress in erythrocytes. ANN analysis showed that optical absorption of hemoglobin at 520, 577, 542, and 630 nm and concentration of metHb and hemichrome were the most important parameters in predicting the oxyHb concentration.

Conclusions: Myrtus communis extract enhances the ability of erythrocytes and plasma to deal with oxidative conditions during exposure to ELFMF. Also, ANN analysis can predict the most important parameters in relation to Hb structure during oxidative stress.

Graphical Abstract

KEYWORDS:

• Extremely low-frequency magnetic fields
• hemoglobin; Myrtus communis
• oxidative stress
• plasma

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Research Council of Arak University of Medical Sciences under Grant Number 2109.

Notes on contributors

Fatemeh Seif

Fatemeh Seif, Ph.D., Assistant professor, Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran.

Mohamad Reza Bayatiani

Dr. Mohamad Reza Bayatiani, Ph.D., Assistant professor, Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran.

Hadi Ansarihadipour

Hadi Ansarihadipour, Ph.D., Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran.

Ghasem Habibi

Ghasem Habibi is a medical student at the Infection Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran.

Samira Sadelaji

Samira Sadelaji, MSc, Department of Immunology and Microbiology, Arak University of Medical Sciences, Arak, Iran.