Nigella sativa oil and thymoquinone reduce oxidative stress in the brain tissue of rats exposed to total head irradiation

Abstract

Purpose: To evaluate the antioxidant and radio-protective effects of Nigella sativa oil (NSO) and thymoquinone (TQ) on radiation-induced oxidative stress in brain tissue.

Materials and methods: Fifty-four Sprague-Dawley rats were divided into six groups to test the radio-protective effectiveness of Nigella sativa oil and thymoquine administered by either orogastric tube or intraperitoneal injection. Appropriate control groups were also studied.

Results: Brain antioxidant capacity, as measured by the levels of total superoxide scavenger activity (TSSA), non-enzymatic superoxide scavenger activity (NSSA), superoxide dismutase, paraoxonase (PON) activities, total antioxidant status and total sulfhydryl (-SH) group, were lower in the irradiation (IR) only group while xanthine oxidase (XO) activity, total oxidant status (TOS), oxidative stress index (OSI) and lipid hydroperoxide (LOOH) levels were higher in the group compared with all other groups. Brain glutathione-S-transferase (GST) activity significantly decreased in the IR only group when compared with the control groups. Glutathione peroxidase (GSH-Px) activity was lower in the IR only, NSO plus IR, TQ plus IR groups when compared with the control group of TQ. Arylesterase (ARYL) activity was not statistically significant in the IR only group compared with all other groups.

Conclusions: The results suggest that Nigella sativa oil (NSO) and its active component, TQ, clearly protect brain tissue from radiation-induced oxidative stress.

Keywords:

• Oxidative stress
• antioxidant
• lipid peroxidation
• free radicals
• irradiation

Disclosure statement

No author has a financial or proprietary interest in any material or methodology referenced.

Additional information

Notes on contributors

Elif Demir

Elif Demir, PhD, Assistant professor of Biochemistry, Harran University, College of Health, Sanlıurfa, Turkey

Seyithan Taysi

Seyithan Taysi, PhD, Professor of Medical Biochemistry, Gaziantep University, Medical School, Gaziantep, Turkey.

Hasan Ulusal

Hasan Ulusal, Research assistant of Medical Biochemistry, Gaziantep University, Medical School, Gaziantep, Turkey.

Davut Sinan Kaplan

Davut Sinan Kaplan, PhD, Assistant professor of Physiology, Medical School, Gaziantep University, Gaziantep, Turkey.

Kadir Cinar

Kadir Cinar, MD, Assistant professor of Neurosurgery, Medical School, Sanko University, Gaziantep, Turkey

Mehmet Tarakcioglu

Mehmet Tarakcioglu, PhD, Professor of Medical Biochemistry, Gaziantep University, Medical School, Gaziantep, Turkey.