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International Journal of Radiation Biology Volume 56, 1989 - Issue 6
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Original Article

Increase in Intracellular Cysteine after Exposure to Dithiothreitol: Implications in Radiobiology

M.F. Dennis Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, PO Box 100, Northwood, Middlesex, HA6 2JR, U.K.
,
M.R.L. Stratford Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, PO Box 100, Northwood, Middlesex, HA6 2JR, U.K.
,
P. Wardman Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, PO Box 100, Northwood, Middlesex, HA6 2JR, U.K.
&
R.R. Watfa Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, PO Box 100, Northwood, Middlesex, HA6 2JR, U.K.
Pages 877-883 | Received 23 May 1989, Accepted 01 Aug 1989, Published online: 03 Jul 2009
 
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Summary

Dithiothreitol reduces cystine in tissue-culture medium, and the resulting cysteine is accumulated in Chinese hamster V79 cells to an extent which should be taken into account in evaluating models of radiosensitivity and the effects of thiol radioprotectors.

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