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Biocontrol Science and Technology Volume 24, 2014 - Issue 2
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SHORT COMMUNICATION

Detection and identification of antibiotic biosynthesis genes in Bacillus subtilis strains

Hadi ZokaeifarScience and Research Branch-Bushehr, Islamic Azad University, Bushehr, Iran
,
Nahid BabaeiScience and Research Branch-Bushehr, Islamic Azad University, Bushehr, Iran;Islamic Azad University Bushehr Branch, Bushehr, Iran
,
Che Roos SaadDepartment of Aquaculture, Faculty of Agriculture, University Putra Malaysia, Selangor, Malaysia
,
Mohd Salleh KamarudinDepartment of Aquaculture, Faculty of Agriculture, University Putra Malaysia, Selangor, Malaysia
,
Kamaruzaman SijamDepartment of Plant Protection, Faculty of Agriculture, University Putra Malaysia, Selangor, Malaysia
&
Jose Luis BalcázarCatalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, Girona, SpainCorrespondence[email protected]
Pages 233-240 | Received 18 Jul 2013, Accepted 03 Oct 2013, Published online: 06 Dec 2013
 
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Abstract

Two Bacillus subtilis strains L10 and G1, previously isolated from fermented pickles, were tested for antibacterial activity against Vibrio harveyi using a well-diffusion agar assay. Antibiotic biosynthesis genes were then detected in both bacterial strains and included the following genes: bacA, bacB and bacD genes for bacilysin production, ppsE/fenB gene for plipastatin/fengycin production, albF and albA genes for subtilosin production, and srfAB and sfP genes for surfactin production. Based on these results, two B. subtilis strains could be considered as potential biological control agents in aquaculture.

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