![Sample our Environment & Sustainability journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5935/TOC-Subject-Sample-2021-Environment-Sustainability.jpg"})
Abstract
A comparative study of three plant peroxidases, horseradish (HRP), soybean (SBP) and artichoke (AKPC), was carried out to select the most appropriate one for 4-chlorophenol treatment in an ultrafiltration membrane reactor. Soybean peroxidase showed the highest enzymatic activity, followed by HRP and AKPC. The same tendency was observed in a discontinuous tank reactor, where SBP attained more than 90% of 4-chlorophenol removal within the pH range tested. The optimum temperature was 30 °C, with SBP showing highest thermostability. With the ultrafiltration membrane reactor, SBP attained the highest operational stability, with 4-chlorophenol conversions of around 90% in the permeate stream for up to 200 minutes. Finally, permeate samples were analysed and no significant amount of enzyme was detected, so the observed loss of activity, less pronounced with SBP, was attributed to enzyme adsorption on the polymeric products deposited on the membrane surface. Soybean peroxidase was selected as the most appropriate peroxidase for future research.
Acknowledgements
This work was supported by 08683/PI/08 research project, financed by the Program of Generation of Scientific Knowledge of Excellence of the Foundation Séneca, Agency of Science and Technology of the Region of Murcia (Spain), in the II PCTRM 2007-10. Also, during the realization of this research, M. Gómez and D.S. Barbosa were beneficiaries of Juan de la Cierva and FPI scholarships, respectively, from MICINN (Ministerio de Ciencia e Innovación). M.D. Murcia and S. Ortega were beneficiaries of Saavedra-Fajardo and pre-doctoral scholarships from Foundation Séneca, respectively.