Acute Taxol Toxicity: the Effects on Bone Marrow Mitotic Index and the Histology of Mice Hepatocytes

Abstract

Rabah, S.O., Ali, S.S., Alsaggaf, S.M. and Ayuob, N.N. 2010. Acute taxol toxicity: the effects on bone marrow mitotic index and the histology of mice hepatocytes. J. Appl. Anim. Res., 38: 201–207.

Hepatotoxic effects of acute intraperitoneal administration of 3 doses; MTD (Maximum tolerated dose) 1.7, ID (Intermediate dose) 1.15 and MD (Medical dose) 0.6 mg/kg Taxol were histologically studied. Mitotic index in hone marrow cells was used to test the drug effect on cell cycle. Vascular congestion, dilation and bile ducts dilatation was observed in liver. Hepatocytes became ballooned and the cytoplasm appeared vacuolated. Apoptotic cells were frequently encountered in all samples. Ultrastructure changes included degeneration of hepatocytes cellular organelles such as endoplasmic reticulum and mitochondria, lipid accumulation and cytoplasmic vacuolation. A significant decrease in mitotic index values in bone marrow cells was observed with higher doses after 24, 48 h. The frequencies of MicroNucleated Polychromatic Erythrocytes (MN-PCEs) were significantly higher in the Taxol treated mice after 24 and 48 h with all doses. This study indicated eneugenic and apoptotic potential of Taxol in mice.

Key words:

• Taxol
• liver
• bone marrow
• mitotic index
• histology