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Abstract
The essential oil of aerial parts of Marrubium duabense Murata (Lamiaceae), growing wild in Salook National Park in northeast of Iran, was obtained by hydro-distillation and the composition was analyzed by GC and GC-MS. 28 components representing 93.4% of total essential oil were identified. The main constituents of the oil were limonene (33.53%), α-terpineol (10.78%), trans-caryophyllene (8.25%), β-elemene (6.37%) and bicyclogermacrene (6.01%). Antibacterial activity was evaluated against four bacteria by the measurement of growth inhibitory zones. The essential oil highly affected the growth of anaerobic strain of Clostridium perfringens.