Profiling Volatile Natural Compounds from Flowers, Leaves and Stems of Marrubium anisodon Using Headspace Solid Phase Microextraction in Combination with Gas Chromatographic Mass Spectrometric Determination

Abstract

The present study describes the potential capabilities of an efficient extraction technique, namely headspace solid-phase microextraction (HS-SPME) to isolate the volatile fractions from the flowers, leaves and stems of Marrubium anisodon in combination with the analysis by GC and GC-MS. By means of the HS-SPME method, 24, 16 and 24 components were characterized in the corresponding profiles, representing 89.6%, 97.4% and 93.6% of the total compositions, respectively. Limonene was the most abundant natural compound in all the volatile profiles. It was also observed that in all of the volatiles, monoterpene hydrocarbons constituted the main components while the next most common group was due to sesquiterpene hydrocarbons for volatile parts of flowers and leaves. However, in that of stems, oxygenated monoterpenes had the second rank from frequency point of view.

Key words:

• Marrubium anisodon
• GC-MS
• Headspace solid-phase microextraction
• Limonene
• Monoterpene hydrocarbons