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Abstract
A galactose biosensor is obtained by immobilizing galactose oxidase (GAO) in a microporous polyacrylonitrile (PAN) thin film. The effects of pH, potential and temperature on response current are studied. The optimum pH and apparent activation energy of enzyme-catalyzed reaction are 7.1 and 31.1 kJ mol−1, respectively. The response current of the biosensor increases linearly with the increasing galactose concentration from 0.02 to 1.60 mmol dm−3. The Michaelis–Menten constant value (Kmapp) is 12.15 mmol dm−3. The biosensor shows good operational stability and reproducibility. The galactose biosensor is characterized with cyclic voltammogram, FTIR and UV-Vis.