DNA Damage, a Biomarker of Carcinogenesis: Its Measurement and Modulation by Diet and Environment

Free radicals and other reactive oxygen or nitrogen species are constantly generated in vivo and can cause oxidative damage to DNA. This damage has been implicated to be important in many diseases, including cancer. The assessment of damage in various biological matrices, such as tissues, cells, and urine, is vital to understanding this role and subsequently devising intervention strategies. During the last 20 years, many analytical techniques have been developed to monitor oxidative DNA base damage. High-performance liquid chromatography-electrochemical detection and gas chromatography-mass spectrometry are the two pioneering contributions to the field. Currently, the arsenal of methods available include the promising high-performance liquid chromatography-tandem mass spectrometry technique, capillary electrophoresis, ³² P-postlabeling, antibody-base immunoassays, and assays involving the use of DNA repair glycosylases such as the comet assay. The objective of this review is to discuss the biological significance of oxidative DNA damage, evaluate the effectiveness of several techniques for measurement of oxidative DNA damage in various biological samples and review current research on factors (dietary and non-dietary) that influence DNA oxidative damage using these techniques.

Keywords:

• reactive oxygen species
• fee radicals
• DNA damage
• cancer
• diet

ACKNOWLEDGEMENT

This work was supported by a Korea Research Foundation Grants (KRF-2004-005-F0055 and KRF-2005-206-F0008) funded by the Korean Government (MOEHRD).

¹Present Address: Center for Agricultural Biomaterial, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-921, Korea.

Notes

^♣Due to the possible artifactual development of 8-OHdG during assay procedure these vales may be overestimated.