Mechanisms Involved in Cardiac Sensitization by Volatile Anesthetics: General Applicability to Halogenated Hydrocarbons?

Abstract

An increased sensitivity of the heart to catecholamines or cardiac sensitization is a recognized risk during acute human exposure to halogenated hydrocarbons used as solvents, foam-blowing or fire-extinguishing agents, refrigerants, and aerosol propellants. Although cardiac sensitization to such “industrial” halocarbons can result in serious arrhythmia and death, research into its mechanistic basis has been limited, whereas the literature on volatile anesthetics (e.g., halothane, chloroform) is comparably extensive. A review of the literature on halocarbons and related volatile anesthetics was conducted. The available experimental evidence suggests that volatile anesthetics at physiologically relevant concentrations interact predominantly with the main repolarizing cardiac potassium channels hERG and I_Ks, as well as with calcium and sodium channels at slightly higher concentrations. On the level of the heart, inhibition of these ion channels is prone to alter both action potential shape (triangulation) and electrical impulse conduction, which may facilitate arrhythmogenesis by volatile anesthetics per se and is potentiated by catecholamines. Action potential triangulation by regionally heterogeneous inhibition of calcium and potassium channels will facilitate catecholamine-induced afterdepolarizations, triggered activity, and enhanced automaticity. Inhibition of cardiac sodium channels will reduce conduction velocity and alter refractory period; this is potentiated by catecholamines and promotes reentry arrhythmias. Other cardiac and/or neuronal mechanisms might also contribute to arrhythmogenesis. The few scattered in vitro data available for halocarbons (e.g., FC–12, halon 1301, trichloroethylene) suggest inhibition of cardiac sodium (conduction), calcium and potassium channels (triangulation), extraneuronal catecholamine reuptake, and various neuronal ion channels. Therefore, it is hypothesized that halocarbons promote cardiac sensitization by similar mechanisms as volatile anesthetics. Experimental approaches for further investigation of these sensitization mechanisms by selected halocarbons are suggested.

Keywords:

• Action potential
• automaticity
• catecholamines
• conduction
• halocarbons
• halothane
• ion channels
• triangulation

ACKNOWLEDGEMENTS

This article evolved from discussions within the ECETOC (European Center for Ecotoxicology and Toxicology of Chemicals) Task Force on “Evaluation of Cardiac Sensitization Test Methods.” Task force members (in alphabetical order) and their affiliations (with e-mail) are: M. Binaglia, Solvay S.A. ([email protected]); C. Chengelis, WIL Research Laboratories ([email protected]); D. Farrar (Chair), Ineos Chlor ([email protected]); C. Hardy, Huntingdon Life Sciences ([email protected]); H.M. Himmel, Bayer HealthCare AG ([email protected]); G. Jepson, DuPont de Nemours (gary.w.jepson–[email protected]); C. Reinhardt ([email protected]); G. Rusch, Honeywell ([email protected]); B. Schmit, Solvay S.A. ([email protected]); H. Vrijhof (Secretary), ECETOC ([email protected]).