Abstract
2’,3’-cyclic nucleotide monophosphates (2’,3’-cNMPs) have been discovered within both prokaryotes and eukaryotes in the past decade and a half, raising questions about their conserved existence in cells. In plants and mammals, wounding has been found to cause increased levels of 2’,3’-cNMPs. Roles for 2’,3’-cNMPs in plant immunity suggest that their regulation may be valuable for both plant hosts and microbial pathogens. In support of this hypothesis, a plethora of microbial enzymes have been found with activities related to these molecules. Studies in bacteria suggest that 2’,3’-cNMPs are also produced in response to cellular stress and modulate expression of numerous genes. 2’,3’-cNMP levels affect bacterial phenotypes, including biofilm formation, motility, and growth. Within E. coli and Salmonella enterica, 2’,3’-cNMPs are produced by RNA degradation by RNase I, highlighting potential roles for Type 2 RNases producing 2’,3’-cNMPs in a range of organisms. Development of cellular tools to modulate 2’,3’-cNMP levels in bacteria has allowed for interrogation of the effects of 2’,3’-cNMP concentration on bacterial transcriptomes and physiology. Pull-downs of cellular 2’,3’-cNMP binding proteins have identified the ribosome and in vitro studies demonstrated that 2’,3’-cNMPs decrease translation, suggesting a direct mechanism for 2’,3-cNMP-dependent control of bacterial phenotypes. Future studies dissecting the cellular roles of 2’,3’-cNMPs will highlight novel signaling pathways within prokaryotes and which can potentially be engineered to control bacterial physiology.
Acknowledgments
The authors are grateful for funding of this work supported by the National Institutes of Health [R01GM125842 to E.E.W.] and Penn State University.
Disclosure statement
No potential conflict of interest was reported by the author(s).