Screening of non-alkaloid acetylcholinesterase and carbonic anhydrase isoenzymes inhibitors of Leiotulus dasyanthus (K. Koch) Pimenov & Ostr. (Apiaceae)

ABSTRACT

The oxidant, cholinesterase, and carbonic anhydrase inhibitions of essential oils and their major compounds of Leiotulus dasyanthus and molecular docking studies of α-pinene and δ-3-carene were evaluated in this study. Phenolic constituents and antioxidant activity of samples were determined by Folin-Ciocalteu’s, DPPH, and TBA processes. Cholinesterase inhibition effect was evaluated through Ellman’s method. GC-FID and GC/MS were used for analysing of essential oils. The root oil got the highest total phenolic content (898.87 mg/g). The utmost activity was shown flower oil (29.31 μg/mL) in the DPPH test. The root oil indicated considerable inhibition towards acetylcholinesterase (21.92%) and butyrylcholinesterase (91.33%) enzymes, respectively. α-pinene and δ-3-carene indicated high acetylcholinesterase inhibitory activity (66.44 and 48.71%, respectively). Cytosolic hCA I and II isoenzymes were influentially inhibited by flower and aerial part oils with 1.694 and 1.677 µM IC₅₀ values, respectively. 1-Dodecanol (7.8%); β-pinene (63.3%), α-pinene (20.5%); octyl acetate (14.7%); hexyl octanoate (55.1%) were characterized as principle compounds of aerial parts, roots, flowers, and fruits respectively.

KEYWORDS:

• Anticholinesterase
• antioxidant
• carbonic anhydrase
• leiotulus
• δ-3-carene
• pinene

Highlights

• Root essential oil showed the highest total phenolic content (898.87 mg/g)

• The highest DPPH activity was revealed in the root essential oil (15.81 mg/g)

• The root essential oil indicated considerable inhibition towards AChE (21.92%)

• The root essential oil indicated considerable inhibition towards BuChE (21.92%)

• α-pinene indicated high butyrylcholinesyterase inhibitory activity 69.45%

• δ-3-carene indicated high butyrylcholinesyterase inhibitory activity 74.77%

• Cytosolic hCA I was inhibited by flower with 1.694 µM

• Cytosolic II was inhibited by aerial part oils with 1.677 µM

Acknowledgments

The authors thanked Prof. Dr Hayri Duman for identifying the plant.

Disclosure Statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was assisted by the Scientific Research Projects of Atatürk University (TAB-2018-6432). Scientific Research Projects of Atatürk University (TAB-2018-6432).